A perusal of the publications in the recent past table 1 shows that various research groups have used widely different protocols which differed in the concentration of dpph 22. In general, the electron transfer et based assays evaluate the capacity of an antioxidant to reduce an oxidant, which usually change color when reduced 24. However, dpph is an expensive chemical especially for under resourced laboratories and potentially 11. If substance for testing antioxidant activity is mixed with dpph solution and gives rise to pale violet, it suggests that this substance has antioxidant effect by mechanism of free radical scavenging activity. Results of the study showed that coconut milk samples exhibited a significantly different p dpph assay provides an easy and rapid way to evaluate antioxidants by spectrophotometry 10, so it can be useful to assess various products at a time. Antioxidant activity of the leaf extract was assessed by dpph, cuprac and pfrap assays using lascorbic acid as standard. Pdf the aim of this study was to assess, using the dpph assay, the antioxidant activity of several substances that could be proposed to.
Dpphfree radical scavenging capacity of legume ex tracts was evaluated according to the method of chen and ho 11 as modified by xu and chang 10. This is the simplest method, wherein the prospective compound or extract is mixed with dpph solution and absorbance is recorded after a defined period. Compare the rates dadt, measured as the slope of a plot versus time. The assay is based on the measurement of the scavenging capacity of antioxidants towards it. Dpph radical scavenging capacity of phenolic extracts from african yam bean sphenostylis stenocarpa 9. Among numerous methods for antioxidant activity estimation, dpph and abts are the most popular and commonly used ones due to their ease, speed, sensitivity and the usage of stabile radicals. Scavenging of dpph radical is the basis of the popular dpph antioxidant assay alma et al. Pdf antioxidant activity by dpph radical scavenging. Kmno 4 is an oxidant like dpph and can be reduced in the presence of antioxidants. Pdf antioxidant activity by dpph radical scavenging method of. The odd electron of nitrogen atom in dpph is reduced by. Taking into account the results of the dpph and abts methods, the free radical scavenging capacity was confirmed.
Use of dpph to estimate antioxidant activity 2 molyneux, p. The ic 50 the concentration of antioxidant which eliminate 50% of dpph. Development and validation of a radical scavenging. Antioxidant activity by dpph assay of potential solutions. They exhibited strong antioxidant dpph radical scavenging activity with ic50 value of 9. Etbased assays encompass one of the most popular antioxidant assays, the dpph.
Dpph radical can accept an electron or hydrogen radical to become a stable diamagnetic molecule and has pale violet. Antioxidant activity was examined by 1diphenyl2picrylhydrazyl. The table 2, 3, and 4 shows the antioxidant activities of the ethanol, methanol and hexane extracts of six green leafy vegetables glvs assessed using the dpph radical scavenging. Antioxidant capacity, radical scavenging kinetics and phenolic. Dpphfree radical scavenging capacity of legume extracts was evaluated according to the method of chen. Dpph radical scavenging activity is one of the most widely used method for screening the antioxidant activity of plant extract. The scavenging activity of the plant extract through the annihilation of the dpph radicals was investigated.
Pdf antioxidant activity by dpph assay of potential solutions to be. Dpph radical scavenging activity of the flavonoids were calculated according to the formula. This method was developed by blois with the viewpoint to determine the antioxidant activity in a like manner by using a stable free radical. The original blois method the dpph method as summarised above was evidently introduced nearly 50 years ago by marsden blois, working at stanford university blois, 1958. Antioxidant activity of methanol extracts of different parts of lantana. Dpph radicalscavenging activity was performed by the method described by akter et al. All the essential oils showed antioxidant activity. Can anyone explain the dpph method for antioxidant.
Is it possible to use the dpph and abts methods for. The absorbance for reducing power was found to be 0. For each determination, the stock solution 1mgml was diluted to a. Conversely, the essential oil of anise in which the percentage of monoterpenes was as low as 2. It is a darkcolored crystalline powder composed of stable freeradical molecules. Characterization and dpph radical scavenging activity of gallic. Pdf methods for determining the antioxidant activity. The dpph assay provides an easy and rapid way to evaluate potential antioxidants. The reduction of dpph radical by antioxidants is evaluated by the. Dpph has two major applications, both in laboratory research. The antioxidant activity of the aerial part extract of m. Original article comparison of abts, dpph, frap, and orac.
Dpph radical scavenging capacity of phenolic extracts from. Screening of in vitro antioxidant activity of methanolic. Comparatively, methanolic extract showed higher activity than ethanolic activity. Antiradical scavenging activity was tested by the dpph model table 5. Dpph free radical scavenging activity of the extracts of. It has been mentioned that antioxidant activity of plants might be due to their phenolic compounds cook and samman, 1996. The extract was screened for possible antioxidant activities by free radical scavenging activitydpph, xanthine oxidase inhibition activity and griess ilosvay. Principle of dpph radical scavenging capacity assay. Radical scavenging activity of plant extracts from improved processing.
Study of antioxidant activity of pyrimidinium betaines by dpph. Antioxidant capacity of selected plant extracts and their. Research further suggests that lipoic acid has a sparing effect on other antioxidants. Dpph assay is based on measurements of the reaction rate between dpph radical with an antioxidant. Among them, thyme and oregano exhibited the highest antioxidant activity, with i dpph values of 98.
The free radical 2, 2diphenyl1picrylhydrazyl dpph method was used for antioxidant assay of methanol plant extracts. Genesis and development of dpph method of antioxidant assay. Total phenols were estimated by folinciocalteus method and flavonoids by. The decreases of the dpph concentration for all samples are shown in fig. Although this paper is short a little over one page in the journal nature. Dpph is a common abbreviation for the organic chemical compound 2,2diphenyl1picrylhydrazyl.
56 136 764 831 1046 1072 110 168 383 987 114 425 605 398 79 533 1376 968 876 1163 281 123 274 128 998 189 2 365 1044 878 511 481 1003 1381 1197 77 17 338 789 1001 104 802 1068 760